Protein
characteristic-
- Polymer consists of monomer amino acids
- C,H,O,N,P,S
- polar ( contains carboxyl group,COOH and Amino group,NH2 at terminal end)
- can be globular and fibrous
- globular: enzymes & hormones
- fibrous: structural proteins(i.e. collagen &keratin)
- 1 or more polypeptide
AMINO ACIDS
- condensation-> polypeptide (held by peptide bond)
- 20 types a.a (different R group)
- different properties ( R group)
- carbon atoms attached to
- amino group
- carboxyl group
- R group
- H atom
5. function determined by specific sequences of a.a. in Polypeptide.
6.zwitterions in water
amphoteric ( can be acidic or basic)
Protein
- primary structure- polypeptide chain formed by unique sequence of amino acid, (peptide bond)
- secondary structure- a-helix &B- pleated P.P chain, - Hydrogen bonding - fold/coil of PP. (repeating configuration)
- Tertiary structure- 3D shape of PP, interaction between amino acids & R group i) ionic bond ii) disulphide bridges iii) Hydrogen bond iv) Hydrophobic & Van der waals interactions
- Quartenary- aggregation of 2 or more polypeptide , (collagen/haemoglobin)
#Protein conformation is determined by chemical& physical conditions in protein environment
# Denaturation= Protein unravels & lose native conformation
causes-i) PH ii) temp iii) salt conc iv) detergant& other chemical which disrupt chemical bonds
