Protein

Protein

characteristic-

• Polymer consists of monomer amino acids
• C,H,O,N,P,S
• polar ( contains carboxyl group,COOH and Amino group,NH2 at terminal end)
• can be globular and fibrous
• globular: enzymes & hormones
• fibrous: structural proteins(i.e. collagen &keratin)
• 1 or more polypeptide

AMINO ACIDS

1. condensation-> polypeptide (held by peptide bond)
2. 20 types a.a (different R group)
3. different properties ( R group)
4. carbon atoms attached to

• amino group
• carboxyl group
• R group
• H atom

5. function determined by specific sequences of a.a. in Polypeptide.

6.zwitterions in water

amphoteric ( can be acidic or basic)

Protein

1. primary structure- polypeptide chain formed by unique sequence of amino acid, (peptide bond)
2. secondary structure- a-helix &B- pleated P.P chain, - Hydrogen bonding - fold/coil of PP. (repeating configuration)
3. Tertiary structure- 3D shape of PP, interaction between amino acids & R group i) ionic bond ii) disulphide bridges iii) Hydrogen bond iv) Hydrophobic & Van der waals interactions
4. Quartenary- aggregation of 2 or more polypeptide , (collagen/haemoglobin)

#Protein conformation is determined by chemical& physical conditions in protein environment

# Denaturation= Protein unravels & lose native conformation

causes-i) PH ii) temp iii) salt conc iv) detergant& other chemical which disrupt chemical bonds